3M E. coli O157 Gebrauchsanweisungen Seite 8

11. Remove the rack of 3M Lysis Solution tubes from the 3M Molecular Detection Chill Block Insert.
Amplification
1. One 3M Molecular Detection Assay 2 - E. coli O157 (including H7) Reagent Tube is required for each sample and the NC.
1.1 Tube strips can be cut to desired tube number. Select the number of individual 3M Molecular Detection Assay
2 - E. coli O157 (including H7) Reagent Tubes or 8-tube strips needed.
1.2 Place 3M Molecular Detection Assay 2 - E. coli O157 (including H7) Reagent Tubes in an empty rack.
1.3 Avoid disturbing the reagent pellets from the bottom of the tubes.
2. Select one 3M Reagent Control tube and place in rack.
3. To avoid cross-contamination, decap one 3M Molecular Detection Assay 2 - E. coli O157 (including H7) Reagent Tube
strip at a time and use a new pipette tip for each transfer step.
4. Transfer lysate to a 3M Molecular Detection Assay 2 - E. coli O157 (including H7) Reagent Tube and 3M Reagent
Control tube as described below:
Transfer each sample lysate into individual 3M Molecular Detection Assay 2 - E. coli O157 (including H7) Reagent
Tubes first followed by the NC. Hydrate the 3M Reagent Control tube last.
5. Use the 3M™ Molecular Detection Cap/Decap Tool-Reagent to decap the 3M Molecular Detection Assay 2 - E. coli
O157 (including H7) Reagent Tube – one tube strip at a time. Discard cap.
5.1 Transfer 20 µL of Sample lysate from the upper ½ of the liquid (avoid precipitate) in the 3M Lysis Solution tube
into corresponding 3M Molecular Detection Assay 2 - E. coli O157 (including H7) Reagent Tube. Dispense at an
angle to avoid disturbing the pellets. Mix by gently pipetting up and down 5 times.
5.2 Repeat step 5.1 until individual sample lysate has been added to a corresponding 3M Molecular Detection Assay 2
- E. coli O157 (including H7) Reagent Tube in the strip.
5.3 Cover the 3M Molecular Detection Assay 2 - E. coli O157 (including H7) Reagent Tubes with the provided extra
caps and use the rounded side of the 3M Molecular Detection Cap/Decap Tool-Reagent to apply pressure in a
back and forth motion ensuring that the cap is tightly applied.
5.4 Repeat steps 5.1 to 5.3 as needed, for the number of samples to be tested.
5.5 When all sample lysates have been transferred, repeat 5.1 to 5.3 to transfer 20 µL of NC lysate into a 3M Molecular
Detection Assay 2 - E. coli O157 (including H7) Reagent Tube.
5.6 Transfer 20 µL of NC lysate into a 3M Reagent Control tube. Dispense at an angle to avoid disturbing the pellets.
Mix by gently pipetting up and down 5 times.
6. Load capped tubes into a clean and decontaminated 3M Molecular Detection Speed Loader Tray. Then close and latch
the lid.
7. Review and confirm the configured run in the 3M Molecular Detection Software.
8. Click the Start button in the software and select instrument for use. The selected instrument's lid automatically opens.
9. Place the 3M Molecular Detection Speed Loader Tray into the 3M Molecular Detection Instrument and close the lid to
start the assay. Results are provided within 60 minutes, although positives may be detected sooner.
00:15:00
99-101ºC
00:05:00
20 µL
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(English)
20-25ºC