3M E. coli O157 Gebrauchsanweisungen Seite 6

NF Validation by AFNOR Certification
For more information about end of validity, please refer to NF VALIDATION certificate available on the website
mentioned above.
NF VALIDATION Certified method in compliance with ISO 16140-2
Scope of the validation: Raw beef meat, raw dairy products, raw fruits and vegetables
Sample preparation: Samples should be prepared according to EN ISO 16654 and EN ISO 6887
Software Version: See certificate
Table 4. Enrichment protocols using pre-warmed BPW ISO at 41.5 ± 1°C according to NF VALIDATION certified method
3M 01/18-05/17
Protocol
Raw dairy products, raw fruits and
raw vegetables
Raw beef meat
NOTES:
• Samples larger than 25 g have not been tested in the NF VALIDATION study.
• The recommended protocol interruption points are after enrichment or after sample lysis. Enrichment broth or sample
lysate can be stored at 2-8°C for up to 72 hours. After removing the enrichment broth from storage, resume testing
from Step 1 in the Lysis section. After removing the sample lysate from storage, resume testing from Step 7 in the Lysis
section. The lysate can also be stored at -20°C.
• Short enrichment protocols are sensitive to incubation conditions and the temperatures specified in the protocol must
be followed. The temperature of the waterbath or the incubator where the broths are prewarmed should be verified to
ensure the enrichment broth is reaching the required temperature. The total time for sample preparation, including the
delay between the end of the medium pre-warming step and the beginning of incubation of the food sample, must not
exceed 45 minutes. Using a ventilated incubator during incubation is recommended.
Preparation of the 3M™ Molecular Detection Speed Loader Tray
1. Wet a cloth or disposable towel with a 1-5% (v:v in water) household bleach solution and wipe the 3M Molecular
Detection Speed Loader Tray.
2. Rinse the 3M Molecular Detection Speed Loader Tray with water.
3. Use a disposable towel to wipe the 3M Molecular Detection Speed Loader Tray dry.
4. Ensure the 3M Molecular Detection Speed Loader Tray is dry before use.
Preparation of the 3M™ Molecular Detection Chill Block Insert
Place the 3M Molecular Detection Chill Block Insert directly on the laboratory bench: The 3M Molecular Detection Chill
Block Tray is not used. Use the block at ambient laboratory temperature (20-25°C).
Preparation of the 3M™ Molecular Detection Heat Block Insert
Place the 3M Molecular Detection Heat Block Insert in a dry double block heater unit. Turn on the dry block heater unit and
set the temperature to allow the 3M Molecular Detection Heat Block Insert to reach and maintain a temperature of 100 ± 1°C.
NOTE: Depending on the heater unit, allow approximately 30 minutes for the 3M Molecular Detection Heat Block Insert
to reach temperature. Using an appropriate, calibrated thermometer (e.g., a partial immersion thermometer, digital
thermocouple thermometer, not a total immersion thermometer) placed in the designated location, verify that the 3M
Molecular Detection Heat Block Insert is at 100 ± 1°C.
3M 01/18-05/17
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
http:/ /nf-validation.afnor.org/en
Sample Enrichment Broth
Size
25 g
25 g
in comparison to ISO 16654
(8)
Enrichment Temperature
Volume (mL)
225
225
5
(English)
(3)
(6)
Enrichment Time
(±1°C) (hours)
41.5 18-24
41.5 8-24