3M E. coli O157 Gebrauchsanweisungen Seite 7

Preparation of the 3M™ Molecular Detection Instrument
1. Launch the 3M™ Molecular Detection Software and log in. Contact your 3M Food Safety representative to ensure you
have the most updated version of the software.
2. Turn on the 3M Molecular Detection Instrument.
3. Create or edit a run with data for each sample. Refer to the 3M™ Molecular Detection System User Manual for details.
NOTE: The 3M Molecular Detection Instrument must reach and maintain temperature of 60°C before inserting the 3M
Molecular Detection Speed Loader Tray with reaction tubes. This heating step takes approximately 20 minutes and is
indicated by an ORANGE light on the instrument's status bar. When the instrument is ready to start a run, the status bar
will turn GREEN.
Lysis
1. Allow the 3M Lysis Solution tubes to warm up by setting the rack at room temperature (20-25°C) overnight (16-18 hours).
Alternatives to equilibrate the 3M Lysis Solution tubes to room temperature are to set the 3M Lysis Solution tubes on the
laboratory bench for at least 2 hours, incubate the 3M Lysis Solution tubes in a 37 ± 1°C incubator for 1 hour or place them
in a dry double block heater for 30 seconds at 100°C.
2. Invert the capped tubes to mix. Proceed to next step within 4 hours.
3. Remove the enrichment broth from the incubator.
4. One 3M Lysis Solution tube is required for each sample and the Negative Control (NC) sample (sterile enrichment
medium).
4.1 3M Lysis Solution tube strips can be cut to desired 3M Lysis Solution tube number. Select the number of individual
3M Lysis Solution tubes or 8-tube strips needed. Place the 3M Lysis Solution tubes in an empty rack.
4.2 To avoid cross-contamination, decap one 3M Lysis Solution tubes strip at a time and use a new pipette tip for each
transfer step.
4.3 Transfer enriched sample to 3M Lysis Solution tubes as described below:
Transfer each enriched sample into an individual 3M Lysis Solution tube first. Transfer the NC last.
4.4 Use the 3M™ Molecular Detection Cap/Decap Tool-Lysis to decap one 3M Lysis Solution tube strip - one strip
at a time.
4.5 Discard the 3M Lysis Solution tube cap – If lysate will be retained for retest, place the caps into a clean container
for re-application after lysis.
4.5.1 For processing of retained lysate, see Appendix A.
4.6 Transfer 20 µL of sample into a 3M Lysis Solution tube unless otherwise indicated in the Protocol Tables 2, 3, and 4.
5. Repeat step 4.3 until each individual sample has been added to a corresponding 3M Lysis Solution tube in the strip.
6. Repeat steps 4.1 to 4.6 as needed, for the number of samples to be tested.
7. When all samples have been transferred, transfer 20 µL of NC (sterile enrichment medium e.g. BPW ISO) into a 3M Lysis
Solution tube. Do not use water as a NC.
8. Verify that the temperature of the 3M Molecular Detection Heat Block Insert is at 100 ± 1°C.
9. Place the uncovered rack of 3M Lysis Solution tubes in the 3M Molecular Detection Heat Block Insert and heat for
15 ± 1 minutes During heating, the 3M Lysis solution will change from pink (cool) to yellow (hot).
.
Samples that have not been properly heat treated during the assay lysis step may be considered a potential biohazard
and should NOT be inserted into the 3M Molecular Detection Instrument.
10. Remove the uncovered rack of 3M Lysis Solution tubes from the heating block and allow to cool in the 3M Molecular
Detection Chill Block Insert at least 5 minutes and a maximum of 10 minutes. The 3M Molecular Chill Block Insert,
used at ambient temperature without the 3M Molecular Detection Chill Block Tray, should sit directly on the laboratory
bench. When cool, the lysis solution will revert to a pink color.
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(English)
20 µl