Hplc Glossary - Knauer Azura DAD 2.1L Benutzerhandbuch

HPLC Glossary

KNAUER
64 HPLC Glossary
In the following chapter you find abbreviations and terminology
that is used in HPLC.
Term Definition
Absorption A substance retaining light of certain wave-
lengths when being shone at.
Adsorption The stationary phase attracting the molecules
of separated substances.
Analytical Qualitative analysis of samples in HPLC (see:
preparative)
Backflush- Flushing of columns and pre-columns by
ing reversing the flow direction. This enables
strongly retarding substances to be separated.
Calibration Process for correcting measuring values by the
value that a measuring device deviates from
the standard.
Capillary Thin metal or PEEK pipe that connects the
components and devices inside of an HPLC sys-
tem.
Carrier Solid particles which carry the stationary phase
material (packing material).
Chromato- The record of a detector signal, depending on
gram the flow volume of the mobile phase or time.
Control Hand-held device with a touchscreen to drive
Unit single AZURA devices locally.
Column The pipe with two end fittings which is perme-
able for solvent. The pipe is filled with packing
material.
Correction Factor that arithmetically corrects device-
factor related deviations from measuring values.
Dead vol- Volume of capillary and system components
ume between mixer, injector and column as well as
between column and detector.
Degasser Degasser module for fluids
Detector Device measuring the composition or the
quantity of a substance
Flow cell Measuring cell
GLP Quality assurance system for laboratories
(Good Laboratory Practice)
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