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If wearing glasses you should remove (18.10,
for 10x/25) or push back (18.10, for 10x/20 and
10x/22) the glare protection, but make sure to
put it on if you are not wearing glasses (18.7).
Set your interpupillary distance by pulling the
eyepiece tubes apart or pushing them closer
together
(Fig.
27)
superimposed image, not a double image, when
you look with both eyes. Make a note of your
personal interpupillary distance, e.g. 65. In
addition for Ergotubes (44.3, 44.5, 46.10): set the
viewing angle (0 – 35°) by tilting the binocular
viewing port. To avoid fatigue symptoms, vary
the viewing angle from time to time.
Filter
Grey filter N/Neutral density filter
Green filter, GR
panchromatic
DLF
BG 38
(blue filter)
ALF
BG 20
VG 9
(green filter)
36
until
you
see
Close any tube exits you will not be using (44.4;
46.5), as stray light may otherwise disturb
viewing.
Analyser*
If the microscope has an integrated analyser
one
(28.1, 32.6), fit or remove as required (only
necessary for polarized light, → p. 46 and
interference contrast → p. 48).

Filters

Light filters (→ Figs. 4 – 6; 21.6, 32.1; 33.7,
"optics" data sheet). Here are the functions of
the most important filters:
Application
Grey filters (neutral density filters) are used to
attenuate light without influencing the colour
temperature. The engraved value, e.g. N16,
indicates the attenuation value. N16, therefore,
means reduction to f
transmission.
Contrast enhancement for black-and-white
photography.
Conversion filter (blue daylight filter, similar to
CB12) for colour photography with daylight film,
integrated in filter magazine.
Suppression
integrated in fluorescence illuminator [39.8]).
Artificial light filter for colour photography with
artificial light film, to enhance colour contrast.
Highlights red in Polaroid exposures.
Contrast enhancement in chromosome
photography.
1
/
= 100/16 = 6.25 %
16
of
red
in
fluorescence
(is

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