Leica DMLB Bedienungsanleitung Seite 55

Move the reflection into the brighter circular
area (40.2 and 40.4), focus (40.3) and align
symmetrically to the direct image (41c).
Alternatively, the images can be made to
coincide, as with Hg and Xe lamps.
Mercury (Hg) and Xenon lamps (Xe)
Move the direct image (41b) to the centre of the
brighter circle with the horizontal and vertical
(40.5 and 40.1) adjustment of the holder. Move
the reflection into the brighter circular area (40.2
and 40.4), focus (40.3) and adjust the mirror until
the reflection coincides with the direct image
(41c).
Caution with He and Xe lamps:
Be careful not to project the reflection on the
electrodes for long, as there is a risk of
explosion if they overheat. The two
electrodes can just be seen in the extension
of the symmetry plane of the discharge arc.
Replace spent burners in good time and
dispose of in an environment-friendly way.
Do not open the lamphousing until the lamp
has cooled down and you have disconnected
it from the mains. Wear protective clothing
(gloves and mask) when using Xe lamps. Hg
lamps take a few minutes to reach their full
intensity; they do not ignite when hot.
Attention:
Never look directly into the light path!
There is danger of glare when switching on
the brightfield reflector (BF) or Smith
reflector (6.4; 6.5)!
Attention:
Reflector, filter systems
Turn an objective into the light path and focus
the specimen in transmitted light first. Select a
reflector to suit the illumination technique (BF/
DF, ICR, POL) or a filter system (16 and 39.2) to
suit the excitation and emission spectrum of the
object (→ optics DM L/DM R data sheet) and
switch it into the light path, assembly → p. 18.
Objective, tube factor
Use high-aperture objectives (immersion) to
obtain optimum fluorescence image intensity;
open the iris diaphragm in the objective if
applicable (Fig. 25.4). Trinocular tube* with
switchable beamsplitter: switch to highest
possible intensity for visual observation (46.4).
For fluorescence:
changer* (45.1), if present, to factor 1x. Protect
the immersion oil from impurities to avoid
disturbing fluorescence. Use low-fluorescence
mounting media, coverglasses and specimen
slides!
Unblock the incident light path (39.9), switch off
transmitted light or cover with slide (39.4; 30.5)
(push into the stage from the front), focus the
specimen. Disengage the BG 38 filter (39.9) if
there is no disturbing red background. Always
engage the filter for photography, however.
Switch magnification
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