Leica DMLB Bedienungsanleitung Seite 37

CB1.5, CB3
CR1.5
BG 23
Filter systems for fluorescence
→ Data sheet for optics
Brightfield, Koehler illumination*
Turn a 10x objective into the light path and focus
the specimen (28.7; 28.8). Condenser UCA/P*
only: engage the condenser top (10.1 and 10.4).
The condenser top is swung out for objective
magnifications < 10x.
Blue conversion filters. Raise the colour
temperature for special lamps.
Red conversion filter. Lowers the colour
temperature, e.g.
temperature of an Xe lamp) to 5500 K (colour
temperature of daylight film).
To enhance the contrast of the complementary
colours blue and red for black-and-white film.
Condensers Field diaphragm*
Close the field diaphragm (28.10).
Narrow the aperture diaphragm (28.14) if
necessary.
Rotate the condenser stop screw (28.5)
clockwise and move the condenser to the
highest position with the height adjustment
(28.4, bilateral). Click the condenser disc* (28.15)
into the "BF" = brightfield position or pull out the
light ring slide* (7.7) as appropriate.
By rotating the condenser stop screw (28.5) or
the condenser height adjustment (28.4), lower
the condenser until the edge of the field
diaphragm appears sharply focused (29b). In
addition, centre the image of the field diaphragm
with both centering screws (28.16a/b), i.e. until it
is in the centre of the field of view (29c).
from 6000 K (colour
37