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Table 4: Enrichment protocols according to NF VALIDATION certified method 3M 01/15-09/16.
Enrichment
Sam-
General
Broth
ple
Protocol
Volume
Size
(mL)
All food
samples
(except
raw meats,
raw
25 g
seafood,
and raw
dairy
products)
25 g,
Environ-
1 swab,
mental
or
samples
1 wipe
Primary Enrichment (Demi-Fraser Broth)
Specific
Sam-
Enrichment
Protocol
ple
Broth Vol-
Size
ume (mL)
Raw
meats, raw
seafood,
25 g
and raw
dairy
products
(a) Volume of sample transferred to Lysis Solution tubes. Refer to Step 4.6 of Lysis section.
Note
• Samples larger than 25 g have not been tested in the NF validation study.
Preparation of the 3M™ Molecular Detection Speed Loader Tray
1. Wet a cloth with a 1-5% (v:v in water) household bleach solution and wipe the 3M™ Molecular Detection Speed Loader
Tray.
2. Rinse the 3M Molecular Detection Speed Loader Tray with water.
3. Use a disposable towel to wipe the 3M Molecular Detection Speed Loader Tray dry.
4. Ensure the 3M Molecular Detection Speed Loader Tray is dry before use.
Preparation of the 3M™ Molecular Detection Chill Block Insert
Place the 3M™ Molecular Detection Chill Block directly on the laboratory bench; (the 3M™ Molecular Detection Chill
Block Tray is not used). Use the chill block at ambient laboratory temperature (20-25°C).
Preparation of the 3M™ Molecular Detection Heat Block Insert
Place the 3M™ Molecular Detection Heat Block Insert in a dry double block heater unit. Turn on the dry block heater unit and
set the temperature to allow the 3M Molecular Detection Heat Block Insert to reach and maintain a temperature of 100 ±1°C.
Note: Depending on the heater unit, allow approximately 30 minutes for the 3M Molecular Detection Heat Block Insert
to reach temperature. Using an appropriate, calibrated thermometer (e.g., a partial immersion thermometer or digital
thermocouple thermometer, not a total immersion thermometer) placed in the designated location, verify that the 3M
Molecular Detection Heat Block Insert is at 100 ±1°C.
Preparation of the 3M™ Molecular Detection Instrument
1. Launch the 3M™ Molecular Detection Software and log in. Contact your 3M Food Safety representative to ensure you
have the most updated version of the software.
2. Turn on the 3M Molecular Detection Instrument.
Enrich-
Enrich-
ment Tem-
ment Time
perature
(±1°C)
225
37
24-30
Enrich-
Enrich-
ment Tem-
ment Time
perature
(±1°C)
225
37
20-24
Recom-
Sample
mended
Analysis
interrup-
(hr)
Volume
(a)
tion point
- Demi-
Fraser
up to 72
hours
20 µL
- lysate at
-20°C,
- lysate
4°C up to
72 hours
Secondary Enrichment (Fraser Broth)
Enrich-
Sample
ment Tem-
Size
perature
(hr)
(±1°C)
Tranfer
0.1 mL
into
37
10 mL
Fraser
Broth
7
(English)
EN
Sample
Enrichment
mended
Analysis
Time (hr)
interrup-
Volume
(a)
tion point
- Demi-
- lysate at
20-24
10 µL
- lysate
Recom-
Fraser
up to 72
hours
-20°C,
4°C up
to 72
hours
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