3M MDA2LMO96 Bedienungsanleitung Seite 2

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Molecular Detection Assay 2 - Listeria monocytogenes

Product Description and Intended Use

3M™ Molecular Detection Assay 2 - Listeria monocytogenes is used with the 3M™ Molecular Detection System for the

rapid and specific detection of Listeria monocytogenes in enriched food and environmental samples.

The 3M Molecular Detection Assays use loop-mediated isothermal amplification to rapidly amplify nucleic acid sequences

with high specificity and sensitivity, combined with bioluminescence to detect the amplification. Presumptive positive

results are reported in real-time while negative results are displayed after the assay is completed. Presumptive positive

results should be confirmed using your preferred method or as specified by local regulations

The 3M Molecular Detection Assay 2 - Listeria monocytogenes is intended for use in a laboratory environment by

professionals trained in laboratory techniques. 3M has not documented the use of this product in industries other than food

or beverage. For example, 3M has not documented this product for testing water, pharmaceutical, cosmetics, clinical or

veterinary samples. The 3M Molecular Detection Assay - 2 Listeria monocytogenes has not been evaluated with all possible

testing protocols or with all possible strains of bacteria.

As with all test methods, the source, formulation and quality of enrichment medium can influence the results. Factors

such as sampling methods, testing protocols, sample preparation, handling, and laboratory technique may also influence

results. 3M recommends evaluation of the method including enrichment medium, in the user's environment using a

sufficient number of samples with particular foods and microbial challenges to ensure that the method meets the user's

criteria.

3M has evaluated the 3M Molecular Detection Assay 2 - Listeria monocytogenes with Demi-Fraser Broth containing Ferric

Ammonium Citrate and Fraser Broth containing Ferric Ammonium Citrate as needed. A typical formulation of this medium

follows below.

Demi-Fraser Broth Base Typical Formula

Sodium Chloride

Sodium Phosphate, dibasic, anhydrous *

Beef Extract

Pancreatic Digest of Casein

Peptic Digest of Animal Tissue

Yeast Extract

Lithium Chloride

Potassium Phosphate, monobasic

Esculin

Acriflavin HCl

Nalidixic Acid

* Substitute: Sodium Phosphate, dibasic, dihydrate 12.0 g

Fraser Broth Supplement

(Ingredients per 10 mL vial. One vial is added to one liter of basal medium.)

Ferric Ammonium Citrate

Final pH 7.2 ± 0.2 at 25°C

The 3M™ Molecular Detection Instrument is intended for use with samples that have undergone heat treatment during

the assay lysis step, which is designed to destroy organisms present in the sample. Samples that have not been properly

heat treated during the assay lysis step may be considered a potential biohazard and should NOT be inserted into the 3M

Molecular Detection Instrument.

3M Food Safety is certified to ISO (International Organization for Standardization) 9001 for design and manufacturing.

The 3M Molecular Detection Assay 2 - Listeria monocytogenes test kit contains 96 tests, described in Table 1.

(g/L)

20 g

9.6 g

5.0 g

3.0 g

1.35 g

1.0 g

0.0125 g

0.01 g

0.5g/10mL

Product Instructions

Fraser Broth Base Typical Formula