3M MDA2LMO96 Bedienungsanleitung Seite 10

Amplification
1. One Reagent tube is required for each sample and the NC.
1.1 Reagent tube strips can be cut to desired tube number. Select the number of individual Reagent tubes or 8-tube
strips needed.
1.2 Place Reagent tubes in an empty rack.
1.3 Avoid disturbing the reagent pellets from the bottom of the tubes.
2. Select 1 Reagent Control (RC) tube and place in rack.
3. To avoid cross-contamination, decap one Reagent tube strip at a time and use a new pipette tip for each transfer step.
4. Transfer lysate to Reagent tubes and RC tube as described below:
Transfer each sample lysate into individual Reagent tubes first followed by the NC. Hydrate the RC tube last.
5. Use the 3M™ Molecular Detection Cap/Decap Tool-Reagent to decap the Reagent tubes–one Reagent tube strip at a
time. Discard cap.
5.1 Transfer 20 µL of Sample lysate from the upper ½ of the liquid (avoid precipitate) in the LS tube into
corresponding Reagent tube. Dispense at an angle to avoid disturbing the pellets. Mix by gently pipetting up
and down 5 times.
5.2 Repeat step 5.1 until individual Sample lysate has been added to a corresponding Reagent tube in the strip.
5.3 Cover the Reagent tubes with the provided extra caps and use the rounded side of the 3M Molecular Detection
Cap/Decap Tool-Reagent to apply pressure in a back and forth motion ensuring that the cap is tightly applied.
5.4 Repeat step 5.1 as needed, for the number of samples to be tested.
5.5 When all sample lysates have been transferred, repeat 5.1 to transfer 20 µL of NC lysate into a Reagent tube.
5.6 Transfer 20 µL of NC lysate into a RC tube. Dispense at an angle to avoid disturbing the pellets. Mix by gently
pipetting up and down 5 times.
6. Load capped tubes into a clean and decontaminated 3M Molecular Detection Speed Loader Tray. Close and latch the
3M Molecular Detection Speed Loader Tray lid.
7. Review and confirm the configured run in the 3M Molecular Detection Software.
8. Click the Start button in the software and select instrument for use. The selected instrument's lid automatically opens.
9. Place the 3M Molecular Detection Speed Loader Tray into the 3M Molecular Detection Instrument and close the lid to
start the assay. Results are provided within 75 minutes, although positives may be detected sooner.
10. After the assay is complete, remove the 3M Molecular Detection Speed Loader Tray from the 3M Molecular Detection
Instrument and dispose of the tubes by soaking in a 1-5% (v:v in water) household bleach solution for 1 hour and away
from the assay preparation area.
NOTICE: To minimize the risk of false positives due to cross-contamination, never open reagent tubes containing
amplified DNA. This includes Reagent Control, Reagent, and Matrix Control tubes. Always dispose of sealed reagent
tubes by soaking in a 1-5% (v:v in water) household bleach solution for 1 hour and away from the assay preparation area.
Results and Interpretation
An algorithm interprets the light output curve resulting from the detection of the nucleic acid amplification. Results are
analyzed automatically by the software and are color-coded based on the result. A Positive or Negative result is determined
by analysis of a number of unique curve parameters. Presumptive positive results are reported in real-time while Negative
and Inspect results will be displayed after the run is completed.
Presumptive positive samples should be confirmed as per the laboratory standard operating procedures or by following
the appropriate reference method confirmation
enrichment broth (if applicable), followed by subsequent plating and confirmation of isolates using appropriate
biochemical and serological methods.
20 µL
, beginning with transfer from the primary enrichment to secondary
(1, 2, 3)
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