3M MDA2LMO96 Bedienungsanleitung Seite 6

Table 2: General enrichment protocols using Demi-Fraser Broth and Fraser Broth at 37 ± 1°C as needed.
Sample
Sample Matrix
Heat-processed,
cooked, cured
meats, poultry,
seafood and fish
Heat-processed /
pasteurized dairy
products
Produce and
vegetables
Multi-component
foods
1 sponge
Environmental
samples
(a)
Raw meat, poultry,
seafood, fish
Sample Matrix
Sample
Raw dairy products
(a) Volume of sample transferred to Lysis Solution tubes. Refer to Step 4.6 of Lysis section.
In AOAC PTM studies, the 3M Molecular Detection Assay 2 - Listeria monocytogenes was found to be an effective method
for the detection of Listeria monocytogenes. The matrices tested in the study are shown in Table 3.
Enrichment
Broth
Size Volume
(mL)
25 g 225
100 or 225
1 swab 10
25 g 475
Primary Enrichment
(Demi-Fraser Broth)
Enrichment
Broth
Size Volume
(mL)
25 g 225
Specific Instructions for Validated Methods
AOAC Official Method
®
AOAC Performance Tested Method
Enrichment
Time (hr)
24-30
24-30
24-30
28-32
Enrichment
Sample Size
Time (hr)
Transfer
0.1 mL into
20-24
10 mL Fraser
Broth
2016.08
SM
# 081501
SM
5
(English)
EN
Secondary Enrichment
(Fraser Broth)
Enrichment Time (hr)
20-24
Sample
Analysis
Volume
(a)
10 μL