Setting The Cultivation Parameters; Operating The Control Elements; Aeration Menu; Aeration With Air - Sartorius Stedim Biotech BIOSTAT RM 20 basic Bedienungsanleitung

4.3.2 Setting the Cultivation
Parameters and Starting
Cultivation
4.4 Operating the Control
Elements

4.4.1 Aeration Menu

4.4.1.1 Aeration with Air

1. Turn the rocker on at the main switch. The main switch is located on the back of
the rocker.
2. Select the size of the CultiBag RM you are using in the CultiBag menu as described
in chapter "4.4.7 Settings Menu" on page 34.
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3. Set the rocker speed as described in the menu
page 29 to ensure visible wave movement. Turn on the rocker. Bei starker
Schaumbildung muss die Wippgeschwindigkeit reduziert werden. Der CultiBag
muss komplett aufgeblasen sein, da sich die Schaumbildung durch Falten oder
Unebenheiten verstärkt.
4. Set the cultivation temperature in the Temperature (T) menu as described in
"4.4.3 Temperature Menu" on page 30.
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Start the heating process after you have made sure that the surface
temperature sensor is properly placed under the CultiBag RM. Otherwise, there
is a danger of overheating. Uniform heating of the culture medium is ensured
when the rocker movements are started before activating the heating panel.
Flow 1 and 2 menu:
There are 2 flow cycles on the reactor that can be controlled independently. The
target value is specified via the "Set" button. The target value can range from 50 ml/
min. to 1000 ml/min. If both flow cycles are active, the max. target value per control
cycle is limited to 500 ml/min. Once set, the "Gasflow control" can then be activated.
The air supply is defined using "supply over." "Process air" means that the reactor is
supplied by external process air (1.0 ... 1.5 bar). Pressing the button switches to the
internal air pump. If this pump is to be used, the user must make sure that no external
compressed air source is connected because this input is used as the intake line for
the pump.
The "Bag Inflation" function can be used to automatically inflate an unfilled bag.
To do this, the correct bag configuration must be specified in the Settings menu.
A timer function is then used to pump the proper amount of air into the bag.
The aeration will also stop when the pressure in the bag reaches 30 mbar.
The red bar indicates the present activity of the valve.
"4.4.2 Speed and Angle Menu" on
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Menu Operation 27