Completing The Process, Cell Harvesting And Shutting Down The Device Under Safe Conditions - Sartorius Stedim Biotech BIOSTAT RM 20 basic Bedienungsanleitung

4.7 Completing the Process,
Cell Harvesting and Shutting
Down the Device under Safe
Conditions
1. Stop the rocker movement as described in section
Menu" on page 29. The bag holder stops automatically at a slightly forward angle so
that the dip tube remains in the medium. For bags with the dip tube at the back,
the rocker can be moved to another stop position as described in section
Position Menu" on page 31.
2. Turn the temperature control off as described in section
Menu" on page 30.
3. Turn the aeration off as described in section
4. Remove the hood from the rocker platform.
5. Clamp off the intake air and exhaust filter.
6. Remove the air intake tubing from the sterile intake air filter.
7. There are several options available for emptying the CultiBag depending on the
process conditions:
y To remove the entire contents via the harvest tubing (with a dip tube), use a
suitable peristaltic pump and hold the CultiBag RM in an upright position until it
is completely empty.
y To release the overpressure, remove the holder with the attached CultiBag RM
from the rocker platform and wait 10–15 minutes until the cells have settled.
Pump out the excess and add fresh medium or move the cell culture to a larger
BIOSTAT RM bioreactor.
®
8. Store the CultiBag RM under the prescribed process conditions before you begin
with downstream processing.
9. Turn off the aeration with the compressed air (if connected).
10. Turn off the main switch of the rocker.
"4.4.2 Speed and Angle
t
"4.4.3 Temperature
t
"4.4.1 Aeration Menu" on page 27.
t
Menu Operation
"4.4.4
t
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