Initial Startup; Overview; Installation Kit; Rocker Design - Sartorius Stedim Biotech BIOSTAT RM 20 basic Bedienungsanleitung

3. Initial Startup

3.1 Overview

3.2 Installation Kit

3.3 Rocker Design

3.3.1 Installing the Bag Holder

3.3.2 Installing the Surface
Temperature Sensor
3. Initial Start-up
The following steps are included in the initial start-up and operation of the
BIOSTAT RM 20 | 50 basic:
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1. Rocker setup
2. Installation and inflation of the CultiBag RM
3. Cultivation start
4. Inoculation, sampling and cultivation process
5. Replacement of the culture medium (optional)
6. Completing the process, cell harvesting and shutting down
the device under safe conditions
7. Cleaning and maintenance
The scope of delivery includes the required connections and connecting pieces.
1. Only use connections and accessory parts for the BIOSTAT
have been approved by Sartorius Stedim Biotech.
2. Only replace connections and accessory parts with products that have been
supplied and/or approved by Sartorius Stedim Biotech.
Malfunctions or defects that are caused by the use of unapproved parts for the
BIOSTAT ® RM 20 | 50 basic are not covered by the warranty.
1. Place the bag holder on the rocker and make sure that it is positioned between
both holding points on the front and back of the basic unit in the appropriate
openings to prevent the holder from shifting.
2. Place the heating mats in the openings of the bag holder. Guide the cable through
the appropriate openings. Connect the right and left cables to the sockets labeled
H1 and H2.
1. Attach the left sensor to the bag holder. Do this by guiding the sensor cable
through the round opening in the center of the heating element. Press down on
the sensor from above until it is attached in the opening via the o-ring. The sensor
is positioned correctly when its silver side is pointing up so that it will later lie
directly on the CultiBag RM as shown in the figure.
Note
If the sensor is positioned incorrectly, this can lead to overheating of the
heating panel. In this case, the heating will stop automatically via a bimetal
trip when the temperature reaches 70 +/-5°C.
2. Now connect the sensor plug to the socket reserved for it on the left side of the
basic unit.
3. Procedure as above for the right PT100 sensor.
RM 20 | 50 basic that
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Initial Startup 21