Cultivation Start; Filling The Cultibag Rm With The Culture Medium - Sartorius Stedim Biotech BIOSTAT RM 20 basic Bedienungsanleitung

4.3 Cultivation Start

4.3.1 Filling the CultiBag RM
with the Culture Medium
26 Menu Operation
Caution!
The internal pump should not be selected if another compressed air source is
connected to the device. If the internal pump is activated and compressed air is
connected to the device, this can cause damage to the pump.
3. Wait until the CultiBag RM has inflated completely and make sure that it is
secured to the bag holder via the lever.
The CultiBag RM should be fully inflated, i.e. there should be no folds or kinks.
Make sure that the exhaust filter is functioning by pressing down gently on
the bag chamber and making sure that air escapes. Once the CultiBag RM is
inflated, the air intake can be shut down when the desired process values have
been reached.
The CultiBag RM should be inflated before being filled with the culture medium
(see section "4.2.5 Inflating the CultiBag RM" on page 25).
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This reduces the foaming of the medium. Regulate the air intake so that the
CultiBag RM remains inflated during filling and does not collapse. The rocker
function should be turned off during filling.
1. Make sure that there is an aseptic connection between the chamber of the
CultiBag RM and the media container by using a BioWelder, Luer connections or
MPC connections to connect both tubes. If you are using Luer or MPC connections,
you must work at a sterile workbench.
To ensure aseptic conditions, the CultiBag RM must be filled at a sterile
workbench if necessary. The Luer connection for sampling can also be used
for this.
2. Pump the desired amount of the culture medium into the bioreactor and make
sure that the media container is also separated from the bioreactor under aseptic
conditions.
Make sure that the tubing clamps of the air intake filer and exhaust filter are
open when filling the medium.
3. Place the hood on the bioreactor.