Malfunctions And Countermeasures - Sartorius Stedim Biotech BIOSTAT RM 20 basic Bedienungsanleitung

7.4 Malfunctions and
Countermeasures
54 Appendix
Problem Possible causes
The CultiBag seems to The CultiBag should
inflate to tightly. inflate so that there
are no folds. It should
also not be too tight
that overpressure
occurs and kinks form
at the hold points.
The CultiBag seems to If the CultiBag RM
be underinflated. is underinflated,
it creates excessive
foaming and poor
mixing. "No airflow"
may appear on the
display.
Extreme foam buildup A certain amount of
in the CultiBag RM foam is normal for the
BIOSTAT
basic. However, the
surface of the medium
should not be covered
with more than 50 %
foam.
Measures
If the bag inflates too tightly, check
that the sterile exhaust filter is not
blocked. Do this by connecting a
tube to the filter and placing the
other end in a container with
water. The filter is blocked if no
bubbles appear. Replace the filter
on a sterile workbench.
In some cases, the return valve to
the sterile exhaust filter may be
blocked.
Unscrew it and replace it with a
new one.
Check the "AIR OUT" output on the
rocker to ensure that aeration is
functioning. The sterile exhaust
filter could be blocked, the closing
clamp could be shut or the aeration
tubing not properly secured.
Replace the exhaust filter on a
sterile workbench or fill the culture
medium into a new CultiBag.
If there is too much foam buildup,
check to make sure that CultiBag
® RM 20 | 50 aeration is functioning properly.
Under inflation can lead to strong
foam buildup. Normally, the foam
will recede after a few hours. If the
foam remains unchanged after this
time, reduce the rocker speed.
However, make sure that it is fast
enough to ensure a sufficient
oxygen supply for the medium.
If there is still too much foam
after this, reduce the rocker angle.
A smaller rocker angle produces
less foam and the rocker speed can
again be increased slightly. For
more information, please contact
your Sartorius Stedim Biotech
representative.