Design And Function; Intended Use Of The Biostat® Rm 20 | 50 Basic; Cultibag Rm - Sartorius Stedim Biotech BIOSTAT RM 20 basic Bedienungsanleitung

1. Design and Function

1.1 Intended Use of the BIO-
STAT RM 20 | 50 basic
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Fig. 1:
Design of the BIOSTAT ® RM 20 basic

1.2 CultiBag RM

1. Design and Function
The BIOSTAT RM 20 | 50 basic is a rocker platform for single-use bioreactors (CultiBag
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RM). The shaft mixing process of this platform uses mechanical energy to ensure
homogenous mixing of cells. The energy supply required for this is achieved via the
low-shear, rocking motion of the rocker to which the CultiBag RM is attached.
This type of cell-friendly motion ensures homogeneous cell distribution in the
cultivation medium whereby the surface of the medium is regenerated without
causing a buildup of bubbles.
The BIOSTAT ® RM 20 | 50 basic is a medium-scale platform with replaceable bag
holders (size 20 or 50) for bags with an operational or cultivation volume of
max. 10 or max. 25 liters. The system can be operated standalone on a laboratory
table or in the incubator. An optional internal aeration unit is available that can
produce an air | CO mixture. Both compressed air and ambient air can be used
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for this. An IR sensor is used to measure the CO
20 | 50 basic can also be combined with the BIOSTAT
which is operated via an intuitive touch screen and controls the process via
single-use sensors. It also includes the software for automatic measured value
recording (MFCS/DA).
If the rocker is connected to the BIOSTAT
"BIOSTAT RM serial port active" appears on the rocker display.
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All target values and control functions can be displayed and controlled via the
BIOSTAT ® RM Tower.
For more information about connecting the rocker to the BIOSTAT
please see the relevant operating instructions.
Cell cultivation takes place in the chamber of the single-use CultiBag RM bioreactor,
which was specially developed for use with the BIOSTAT
RM come gamma-sterilized and ready for use. The film that the CultiBag RM is made
of corresponds to the USP Class VI requirements. The volume data for the CultiBag
RM always refers to the gross volume. The maximum operating volume is 50 % of the
gross volume because the remaining space acts as the headspace. The CultiBag RM is
designed for single-use. This does away with the cleaning and sterilization required
for a conventional bioreactor, saving considerable effort and labor. Thanks to the
single-use design of the CultiBag RM, cross-contamination can be prevented and
process reliability increased.
The BIOSTAT RM 20 | 50 basic is designed to only use CultiBags RM.
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concentration. The BIOSTAT
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RM Tower control unit,
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RM Tower it can be controlled by it.
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® RM Tower,
® RM 20 | 50 basic. CultiBags
Design and Function
RM
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