Multi Wavelengths Mode; Measurement Principle; Calculation Examples; Method Programming - Sl Analytics UviLine Sl 5000 Gebrauchsanleitung

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8 Multi wavelengths mode

8.1 Measurement principle

This measuring mode allows making different calculations on a sample measured at several wavelengths
(ratio, Allen correction, ...).
It also makes it possible to have the absorbance value of a solution read at several wavelengths (up to 10).

8.2 Calculation examples

Determination of DNA purity index
DNA purity index is given by the ratio of absorbance read at 260 nm on the absorbance read at 280 nm with
elimination of a cloudy part of the solution by a measurement at 320 nm.
Equation 1
Warburg Christian formula for proteins quantification
Equation 2
Allen correction
It allows measuring the pick high inside a first order chemical noise.
Equation 3

Abs

8.3 Method programming

The programming of a Special-Multi wavelength method is described by the Warburg Christian equation as an
example.
<HOME>
Special/Multi wavelengths
– [Setup]
- New Method
- If necessary, come back to the main menu with <HOME> key.
- Select with « ▲ » and « ▼ » keys, « Special Multi-wavelengths » mode.
- Enter in the multi-wavelengths mode.
Multi wavelengths mode
Abs

R
Abs
 
C , 1 ( 55 Abs
 
Pr oteins

Abs 1

Abs 2

Abs
260 nm 320

Abs
280 nm 320
 
) ( , 0 575 Abs
280 nm

 
Abs 3 2 Abs

2
nm
nm
)
given in mg/mL
260 nm
 
2 Abs 1 Abs
2
3