R-Biopharm RIDAGENE CMV PG9005 Bedienungsanleitung Seite 36

11.2.2 Conversion to genome equivalents/ml
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For quantitative evaluation of the RIDA GENE CMV real-time PCR, the DNA content
of the parameter is identified in genome equivalents/reaction. A factor is used to
convert the specimen to genome equivalents/ml, as specified in Table 9. The
conversion takes into account the dilutions of the DNA extraction (depending on the
device used) and the PCR approach.
Table 9: Conversion to genome equivalents/ml
Quantification of extracted specimens using MagNA Pure 96
Formula for quantifying EDTA plasma specimens
Nc (gEq/ml) = 12.5 x quantity
Nc = CMV concentration in gEq/ml of the specimen used in the extraction
gEq = Genome equivalent
Quantity = CMV gEq per reaction, cycler indication
11.2.3 Conversion to international units (IU)
To convert a quantitative result from gEq/ml to international units/ml, use the
following conversion factor. It takes into account the dilutions of the DNA extraction
(depending on the extraction device used), the PCR preparation, and the traceability
to the WHO standard.
Table 10: Conversion to international units/ml
Conversion to international units/ml of extracted specimens using
MagNA Pure 96 (Fc = 0.54 IU/gEq)
Formula for quantifying plasma specimens (EDTA)
Nc (IU/ml) = 6.75 x quantity
Nc = CMV concentration in IU/ml of the specimen used in the extraction
gEq = Genome equivalent
Quantity = CMV gEq per reaction, cycler indication
Fc = Conversion factor
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2019-05-28
36 RIDA GENE CMV