R-Biopharm RIDAGENE CMV PG9005 Bedienungsanleitung Seite 27

5. Storage instructions
All reagents must be stored away from light at -20 °C and, if unopened, can be used
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until the expiration date printed on the label. After the expiration date, the quality
guarantee is no longer valid.
All reagents should be carefully thawed prior to use (e.g., in a refrigerator at
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2 - 8 °C).
Repeated freezing/thawing of up to 5 times does not have an impact on the test
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property (if necessary, create aliquots after the first thaw and freeze reagents
immediately).
Cool all reagents during PCR preparation (2 - 8 °C).
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6. Additional necessary reagents and necessary equipment
®
The RIDA GENE CMV real-time PCR test can be used with the following extraction
platforms and real-time PCR instruments:
Table 2: Validated devices
Extraction platforms
Promega
Roche
Real-time PCR instruments
Roche
Agilent Technologies
Bio-Rad
QIAGEN
Note: If using Rotor-Gene Q (QIAGEN), use only 0.1-ml reaction vials.
Should you have to use other extraction procedures or real-time PCR devices,
please contact R-Biopharm to check the compatibility at mdx@r-biopharm.de.
Real-time PCR consumables (plates, reaction vials, films)
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- Centrifuge with rotor for reaction vial or plates
Vortexer
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Pipettes (0.5 - 20 µl, 20 - 200 µl, 100 - 1000 µl)
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Pipette tips with filters
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Powder-free disposable gloves
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PCR water (BioScience-grade, nuclease-free, DEPC-treated water, Roth)
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RIDA GENE CMV
®
Maxwell RSC
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Maxwell RSC Viral Total Nucleic Acid
Purification Kit AS1330
MagNA Pure 96
MagNA Pure 96 DNA and Viral NA
Small Volume Kit 06543588001
®
LightCycler
Mx3005P
CFX96™
Rotor-Gene Q
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