Sartorius Sartobind Rapid A Lab Gebrauchsanleitung Seite 20

Storage
Chromatography Recommended values for
step
Buffer
Loading Not applicable
Washing Commonly used buffers for equili-
bration | washing, e.g., 1 x PBS pH 7.4
Elution pH 2.5 – 4, e. g., 50 – 100 mM citric
acid, acetic acid, or glycine
Washing (optional) Commonly used buffers for equili-
bration | washing, e.g., 1 x PBS pH 7.4
Regeneration 0.1 – 0.2 M NaOH
Re-equilibration Commonly used buffers for equili-
bration | washing, e.g., 1 x PBS pH 7.4
20 Instructions for Use Sartobind
5 .4 .3 Performing a Purification Cycle
Materials required:
Requirements
— If operating with inline prefiltration: The microfilter is installed on the LC
system and connected to the inlet of the product.
— The product is equilibrated.
Procedure
Start the purification manually or with an automated program, with the
t
recommended volumes and flow rates (see table above and the instruc-
tions of the LC system).
When using inline prefiltration: If any pressure increase is detected
t
during sample loading, replace the prefilter.
6 Storage
Requirements
The product is re-equilibrated.
Procedure
Fill the product with storage buffer (see chapter "9.6 Storage Condi-
t
tions", page 25).
Install the protective caps on the product inlet and product outlet.
t
Rapid A Lab
®
— Buffers
— Plates or tubes to collect eluate fractions
Volume
Not applicable
10 mL | 20 MV
5 mL | 10 MV
10 mL | 20 MV
5 mL | 10 MV
10 mL | 20 MV
Flow Rate
2.5 – 15 mL/min |
5 – 30 MV/min
≤ 25 mL/min |
≤ 50 MV/min
2.5 – 15 mL/min |
5 – 30 MV/min
≤ 25 mL/min |
≤ 50 MV/min
5 mL/min | 10 MV/min
≤ 25 mL/min |
≤ 50 MV/min