Sartorius Sartobind Rapid A Lab Gebrauchsanleitung Seite 15

5 .3 Using a Peristaltic Pump
5 .3 . 1 Preparing the Peristaltic Pump
Materials required: — Sartobind
— ≥ 10 mL equilibration buffer, e.g. 1 x PBS pH 7.4
— Beaker | tube to collect flow-through
1
2
Fig . 2: Sartobind Lab pump tubing
®
Pos . Name
1 Tubing
2 Male Luer-Lock
connector
Procedure
Prepare the peristaltic pump for purification (see instructions for the per-
t
istaltic pump).
Load the tubing into the peristaltic pump.
t
Fill the tubing with equilibration buffer. Make sure the tubing is free from
t
air .
5 .3 .2 Installing and Equilibrating the Product
Materials required: — Optional: 0.2 µm microfilter for inline prefiltra-
— > 10 mL equilibration buffer, e.g. 1 x PBS pH 7.4
— Beaker | tube to collect flow-through
Lab pump tubing (see chapter "10.2
®
Accessories", page 26)
Description
Transfers buffers and the sample from a ves-
sel (e.g. a beaker) to the product.
Connects the pump tubing to the product
inlet .
tion (see chapter "9.7 Approved Microfilters",
page 25)
Instructions for Use Sartobind
Operation
Rapid A Lab 15
®