Capillary Connection To An Hplc System; Sop 1 - Knauer Smartline 2300 Benutzerhandbuch

Operating the RI Detector 2300/2400

Capillary Connection to an HPLC System

SOP 1

Flow Schemes
Fig. 9
Before placing the detector into operation, please make certain that
the eluent which will be used is miscible with the one used
previously. Otherwise purge the flow cell with a medium miscible
with both of the eluents.
Even though the Smartline RI Detector 2300/2400 is very resistant to
many kinds of commonly used eluents, you should take care that no
eluent or water can get on the instrument's surface or even inside
the instrument. Chlorinated hydrocarbons could destroy the varnish
and some others (e.g. THF) could loosen the keypad, for example.
Connecting Capillaries
1. Connect the outlet of the HPLC column to the inlet bushing (IN) of
the detector.
2. Push the bushing, the clamping ring, and the sealing ring onto the
capillary (for standard connections just use the respective bushing
and clamping ring). Please take care to note the sequence and
orientation of the fittings according to Fig. 12
3. Push the capillary as far as possible into the detector's inlet.
4. Fasten the bushing by hand (standard connection: please use the
supplied wrench).
5. Connect the detector's outlet (OUT) using a capillary or PTFE tube
(ID > 1 mm) to a waste bottle.
Make sure that the detector's capillary input and output connectors
(IN/OUT) are not mixed up with each other; otherwise the cell might
be damaged.
The flow schemes of both RI detectors are shown in the following figures.
optical bench
ref
sig
Flow Scheme of Smartline RI Detector 2300
valve block
ref
sig
.
IN
OUT
17