Knauer Smartline 2300 Benutzerhandbuch Seite 19

18 Operating the RI Detector 2300/2400
IN
OUT
optical bench
valve block
ref
ref
sig
sig
Fig. 10 Flow Scheme of Smartline RI Detector 2400
Capillary Connections
The capillary connections in a simple HPLC system are shown in the
following figure.
The measuring cell is made of glass and hence it is very pressure
sensitive. The pressure inside the cell must not exceed 2 bar at any
time. Higher pressure might destroy the cell. The RI detector should
always be the last instrument in an HPLC system. Make sure that no
back pressure is built up on the outlet side.
Usage of well degassed eluents is very important for achieving
good chromatograms with an RI detector. Degassing ensures a
stable baseline and high sensitivity.