Cleaning The Flow Cell; Analytical Flow Cells; Sop 9 Purging The Flow Cell; Sop 10 Cleaning An Analytical Flow Cell - Knauer Smartlibe UV Detector 2500 Handbuch

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Cleaning the flow cell

SOP 9

Analytical flow cells

Fig. 30
SOP 10
7. Insert the socket with lamp into the instrument, screw it in securely ,
and connect the 2-pole plug into the 2-pole socket.
8. Increase the lamp counter in the GLP-menu. The operating time
counter will be set to zero automatically.
9. Put the optical bench carefully back into the housing.
10. Switch the detector on again and check the intensity values in the
INTENSITY menu.
Noisy baselines and low sensitivities may be due to a dirty flow cell. This
may also be indicated by a low value for signal in the signal menu when
flushing the cell with pure solvent. In most cases it is sufficient to purge
the flow cell according to the following SOP.
Purging the Flow Cell
1. Purge the flow cell using one of following solvents: sodium dodecyl-
sulfate (SDS), 1m HCl, 1m NaOH, ethanol, or acetone.
2. Run the solvent through the flow cell using a syringe and leave it in
the cell for approximately 5 minutes.
3. Rinse extensively with water and then blow dry using a gentle stream
of pure nitrogen.
Never dry with compressed air from a „house" line as this will contain
microdroplets of oil that will coat the cell.
When the cell is not in use, disconnect the flow cell and clean out traces of
salt and protein with a syringe filled with distilled water. Before storing the
flow cell inject a dilute solution (10-25%) of ethanol or iso-propanol to
prevent microbial growth.
In case flow cell purging does not provide sufficient success, all flow cells
can easily be disassembled for cleaning the lenses for example in an
ultrasonic bath.
Sectional view of an analytical flow cell
Cleaning an analytical flow cell.
This instruction applies to the analytical flow cell A4061.
1. Unscrew the outer threads with the 3 mm hexagonal spanner enclosed
in the flow cell's delivery.
2. Remove the black gasket that carries the lenses with a pair of
tweezers or by gently tapping it on a clean surface. The lens is
Simple Maintenance
Outer thread
Gasket
Lens
PFA Sealing