Rs-232 Serial Interface; Interface Protocol; Scanning - Knauer Smartlibe UV Detector 2500 Handbuch

22

RS-232 Serial Interface

Fig. 19

Interface protocol

Scanning

SOP 4
This chapter gives only a short information regarding the connections of
the detector when working with the UV-Detector 2500 under EuroChrom
®
or ChromGate software control. For more detailed information
concerning the features of the software, please consider the user manual
of the software.
Working under software control, the PROGRAM menu must be
empty, no program is allowed! Only then a START signal will be
interpreted as command to start the measurement. In case a
program is entered, any START signal will be interpreted as
command to start this program.
The RS-232 serial interface on the rear side of the device, item 4 in Fig. 9
on page 10, enables digital data transfer between the UV-Detector 2500
and a PC, equipped with HPLC software (EuroChrom
Please connect this interface directly or if necessary via an interface
multiplier to a COM-Port of your computer. In addition the start contact of a
manual injection valve or an autosampler may be connected to START and
GROUND of the remote control socket.
RS-232 interface and remote control socket
To operate the UV-Detector controlled by a software package via RS-232
set EXTERNAL. CONTROL to keyboard (see page 14).
To control the Smartline UV-Detector 2500 with a non-KNAUER software
via RS-232 you can get the interface protocol from KNAUER upon
request.
During a chromatographic run you can scan a received peak in a stopped
flow mode. You can select the wavelength range which is to be scanned
in steps of 2 nm. Also you can select the scan rate 1 Hz, 3 Hz (standard)
or 6 Hz. An additional option is 0 Hz to perform every step manually by
pressing one of the ▲ or ▼ keys.
To perform a scan proceed according to SOP 4
Scanning
This instruction refers to the UV-Detector 2500.
1. Stop the eluent delivery of your HPLC pump (flow = 0).
2. Move to the SCAN menu by using the ▲ or ▼ keys.
3. Enter the scan range beginning with the lower wavelength.
4. Activate (+F) or deactivate (-F) the edge filter.
Operating the UV-Detector 2500
®
or ChromGate
®
®
).