Knauer Smartlibe UV Detector 2500 Handbuch Seite 24

Operating the UV-Detector 2500
Never use the filter below 380 nm because you would not get any
results.
If you need a scan starting below and ending above 380 nm and the upper
range shall be scanned with active filter it is recommended to take two
separate partial scans.
5. Enter in the speed field the scan speed 1 Hz, 3 Hz, 6 Hz or the
manual option 0 Hz.
6. To perform an AUTO-ZERO scan position the cursor on the AZ field
and press one of the ▲ or ▼ keys.
The scan AUTO-ZERO has to be carried out prior to each scan.
Otherwise you would get only the reference signals instead of the
absorption ones.
7. When the substance is in the flow cell the pump is stopped again
and the scan is executed by pressing one of the ▲ or ▼ keys with
the cursor on the go field.
During a running scan the display looks like below with steadily updated
values for both the signal and the wavelength.
0.1 s 0.0000 au
♦ ON
Please note that a spectrum is only printed with a time axis even when it
is recorded by a chromatography software. Though it is always necessary
to convert to wavelength by linear interpolation between start- and end-
point of the spectrum.
If you are performing a scan manually (speed = 0 Hz), the display shows
the additional hint move with a blinking cursor on the m.
0.1 s 0.0000 au
♦ ON move
Then you will get the next wavelength by pressing one of the ▲ or ▼
keys.
Pressing one of the ► or ◄ keys will abandon any scan
independent on the scan speed selected.
254 nm
254 nm
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