Capillary Connection To An Hplc System; Sop 3 Capillary Connections - Knauer Smartlibe UV Detector 2500 Handbuch

18

Capillary Connection to an HPLC System

SOP 3
Fig. 15
Before taking a measurement cell filled with solvent into operation,
please make certain that the used eluent is miscible with that one
used previously. Otherwise purge the flow cell with a medium
miscible with both the eluents.
Capillary connections.
This instruction refers to the UV-Detector 2500.
1. Connect the outlet of the HPLC column to the inlet bushing of the flow
cell (lower side).
2. Push the bushing, the clamping ring, and the sealing ring onto the
capillary (for standard connections just use the respective bushing
and clamping ring). Please take care on the sequence and orientation
of fittings, see Fig. 16 „DYNASEAL Capillary connections".
3. Push the capillary as far as possible into the flow cell inlet.
4. Fasten the bushing by hand (standard connection: please use the
supplied wrench).
5. Connect the flow cell outlet (upper side) using a capillary or PTFE
tube (ID > 0,5 mm) to a waste bottle.
The capillary connections in a simple HPLC system are shown in the
following figure.
Capillary connections of the detector
(example: Low pressure gradient HPLC System)
Operating the UV-Detector 2500