Materials Required But Not Provided; Assay Protocol; Warnings And Precautions; Collection And Preparation Of Blood Samples - peviva M30 Apoptosense ELISA Gebrauchsanweisung

Materials Required but not Provided

• Microplate reader (wavelength: 450 nm; linear 0–3 OD)
• Microplate shaker (oscillation: 600 rpm, orbit: 1.5–4 mm)
• 96-well microtiter plate washer or multichannel pipette (volume 250 µL)
• Vortex mixer
• Precision pipettes: 25, 50, 75 and 200 µL
• Cylinder (500 mL)
• Deionised water

Assay Protocol

Warnings and Precautions

1. M30 Apoptosense ELISA kit is intended for in vitro use only.
2. Do not mix reagents from different kit lots.
3. All patient specimens should be regarded as contagious and handled and
disposed of according to appropriate regulations.
4. Do not use samples that are contaminated.
5. The Stop Solution contains 1.0 M sulphuric acid, which will cause irritation
of the skin and is harmful to the eyes. In case of contact, flush with plenty
of water and seek medical advice.
6. Material Safety Data Sheets (MSDS) are available on www.vlvbio.com or
by request.

Collection and Preparation of Blood Samples

The sample volume should be sufficient for measuring each sample in
duplicate (test volume 2 × 25 µL). Donors do not need to be fasting prior to
blood collection.
Serum: Collect blood by venipuncture, avoiding haemolysis, into plain
tubes (without anti-coagulant), allow blood to clot and collect serum after
centrifugation.
Plasma: The M30 Apoptosense ELISA can also be used for plasma samples
(EDTA or heparin).
Note!
The same type of material, i.e. serum or plasma collected by one
method, should be used for a specific project. For further information on the
performance of the M30 Apoptosense ELISA using different types of samples,
please consult www.vlvbio.com.
M30 Apoptosense® ELISA
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