Materials Provided For 96 Determinations - peviva M30 Apoptosense ELISA Gebrauchsanweisung

Instructions for Use
By plotting a standard curve from known concentrations versus measured
absorbance, the amount of antigen in the sample can be calculated. The
concentration of the antigen is expressed as units per litre (U/L).

Materials Provided for 96 Determinations

M5 Coated Microstrips: One microplate, 12 strips with 8 wells each, 96 dry wells
in total. The wells are coated with mouse monoclonal K18 antibody M5. The
microplate is sealed in an aluminium bag, which contains a desiccating device. If
not all the strips are used, reseal the bag and keep the desiccating device inside.
Ready for use!
M30 Conjugate: Concentrate (24 × conc). One vial containing 0.4 mL of mouse
monoclonal M30 antibody (anti-K18Asp396 neo-epitope) conjugated with
horseradish peroxidase (HRP) in phosphate buffer with protein stabilizers.
Preservative added. Should be diluted with M30 Conjugate Dilution Buffer.
Note!
Do not expose to light!
M30 Conjugate Dilution Buffer: One vial containing 12 mL of phosphate buffer
with protein stabilizers for dilution of the M30 Conjugate. Preservative added.
Blue coloured.
M30 Standard A – G: Standard A containing 2.5 mL of phosphate buffer with
foetal calf serum (FCS). Standard B – G, 0.6 mL each, containing standard material
in phosphate buffer with FCS. The values of Standard A – G are 0, 75, 150, 250,
500, 750 and 1 000 U/L, respectively. Preservative added. Yellow coloured. Ready
for use! Standard A can be used for dilutions of samples > 1 000 U/L.
M30 Control Low & High: Two vials containing 0.6 mL of reactive components
in phosphate buffer with FCS. The values of M30 Control Low and M30 Control
High are stated on the respective vials. Preservative added. Yellow coloured.
Ready for use!
Wash Tablet: One tablet for 500 mL of prepared wash solution. Dissolve the
Wash Tablet in 500 mL of fresh deionised water.
TMB Substrate: One bottle containing 22 mL of TMB (3,3' , 5,5'-Tetramethyl-
Note! Ready for use!
benzidine) Solution. Do not expose to light!
Stop Solution: One vial containing 8 mL of 1.0 M sulphuric acid. Ready for use!
Sealing Tape: One (1) sheet.
Instructions for Use.
Certificate of Analysis.
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