Operation; Calibration Of The Co Sensor - Mettler Toledo INGOLD InPro 5000 Bedienungsanleitung

InPro 5000 (i) CO
5

Operation

5.1 Calibration of the CO
The InPro 5000 (i) sensor can be calibrated with CO
gas or a CO
partial pressure of 150 mbar is expected, you can use
a gas with 15 % CO
best results. The calibration can be made in a gas or
liquid phase. For calibration in a solution you have to
wait until an equilibrium is reached. Depending on the
pH value of the solution a large amount of CO
react to build bicarbonate ions before building-up a
constant partial pressure of CO
h
Recommendations:
– For small "benchtop" bioreactors the sensor can
be calibrated by flushing the empty or liquid
filled reactor with a CO
– For larger bioreactors the gas consumption would
be too high and therefore we recommend the
calibration with the aid of the retractable
housing InTrac 797. Please operate the retract-
able housing according the instruction manual
InTrac 797 to ensure sterile operation. Additionally
the in- and outlet of the lower flushing chamber
must be equipped with sterile filters to avoid any
contamination with the calibration gas.
Calibration procedure:
– Sterilize CO
position (membrane inside bioreactor) together
with the bioreactor.
– Sterilize lower and upper flushing chambers
of housing InTrac 797.
– After cooling down retract sensor in maintenance
position (membrane in lower chamber).
– Flush lower chamber with sterile calibration gas.
The pressure inside the flushing chamber must be
known for proper calibration.
– After calibration close in- and outlet of the
lower chamber and reinsert sensor in measuring
position. Sensor is now ready for measurement.
© 03 / 2023 METTLER TOLEDO
Printed in Switzerland
Sensor 12 mm
2
/ N gas mixture. Example: If a CO
2 2
/ 85 % N
2
sensor InPro 5000 (i) in measuring
2
sensor
2
in nitrogen to achieve
2
.
2
containing gas mixture.
2
15
will
2
InPro 5000 (i)
52 002 465 G
2
2