Operation; Calibration Of The Co Sensor - Mettler Toledo InPro 5000 Bedienungsanleitung

52002465_F_IM_InPro5000i_edf_2022_09.qxp_52002465_F_IM_InPro5000_edf_2022
InPro 5000 (i) CO
5

Operation

5.1 Calibration of the CO
The InPro 5000 (i) sensor can be calibrated with CO
gas or a CO
partial pressure of 150 mbar is expected, you can use
a gas with 15 % CO
best results. The calibration can be made in a gas or
liquid phase. For calibration in a solution you have to
wait until an equilibrium is reached. Depending on the
pH value of the solution a large amount of CO
react to build bicarbonate ions before building-up a
constant partial pressure of CO
Recommendations:
• For small "benchtop" bioreactors the sensor can
be calibrated by flushing the empty or liquid
filled reactor with a CO
• For larger bioreactors the gas consumption would
be too high and therefore we recommend the
calibration with the aid of the retractable
housing InTrac 797. Please operate the retractable
housing according the instruction manual
InTrac 797 to ensure sterile operation. Additionally
the in- and outlet of the lower flushing chamber
must be equipped with sterile filters to avoid any
contamination with the calibration gas.
Calibration procedure:
• Sterilize CO
position (membrane inside bioreactor) together
with the bioreactor.
• Sterilize lower and upper flushing chambers
of housing InTrac 797.
• After cooling down retract sensor in maintenance
position (membrane in lower chamber).
• Flush lower chamber with sterile calibration gas.
The pressure inside the flushing chamber must be
known for proper calibration.
• After calibration close in- and outlet of the
lower chamber and reinsert sensor in measuring
position. Sensor is now ready for measurement.
© 09 / 2022 METTLER TOLEDO
Printed in Switzerland
Sensor 12 mm
2
2
/ N gas mixture. Example: If a CO
2
2
/ 85 % N
2
sensor InPro 5000 (i) in measuring
2
sensor
in nitrogen to achieve
2
.
2
containing gas mixture.
2
InPro 5000 (i)
52 002 465 F
15
2
2
will
2