GE Ettan IPGphor 3 Sicherheitshandbuch Seite 15

Ettan IPGphor 3 Safety Handbook 11-0034-61 Edition AB
1.4.2 IPG strips, strip holders, and reagents
CAUTION! The ceramic strip holders and manifold trays are very brittle and
fragile. Handle carefully: Do not bump or drop.
CAUTION! Always wear gloves when handling IPG strips and the equipment
that comes in contact with them. This will help minimize protein
contamination which can result in artifactual spots in stained 2-D gel patterns.
CAUTION! Clean strip holders and manifold with the strip holder cleaning
solution provided. Clean all other components that come in contact with the
IPG strip or the sample with a detergent designed for glassware. Rinse well
with distilled water.
CAUTION! Use the appropriate rehydration volume for the IPG strip length.
CAUTION! Solutions containing urea may be warmed briefly. Do not heat any
solutions containing urea above 30–40 ºC as isocyanate, a urea degradation
product, will carbamylate the proteins in the sample, thus changing their
isoelectric points.
CAUTION! All chemicals should be of the highest purity. Double distilled water
should be used.
Safety information
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