tecan SPARK CYTO300 Gebrauchsanweisung Seite 7

8 Betrieb des SPARK mit SparkControl Software ................................................................. 53
8.1 Anwendungsgebiet.......................................................................................................................... 53
8.2 Systemanforderungen ..................................................................................................................... 53
8.3 Installation der Software .................................................................................................................. 55
8.3.1 Deinstallieren/Installation reparieren .................................................................................. 55
8.3.2 IoT Client ........................................................................................................................... 56
8.4 SparkControl starten ....................................................................................................................... 57
8.4.1 Geräte verbinden ............................................................................................................... 57
8.5 Methoden Editor .............................................................................................................................. 57
8.5.1 Struktur .............................................................................................................................. 57
8.6 Dashboard ...................................................................................................................................... 60
8.6.1 Struktur .............................................................................................................................. 60
8.6.2 Dashboard ......................................................................................................................... 62
8.7 Eine Methode starten ...................................................................................................................... 65
8.7.1 Methoden Editor................................................................................................................. 65
8.7.2 Dashboard ......................................................................................................................... 65
8.7.3 Starten mithilfe der Tasten am Gerät ................................................................................. 65
8.8 Einstellungen der SparkControl ...................................................................................................... 66
8.8.1 Struktur .............................................................................................................................. 66
8.9 Messergebnisse .............................................................................................................................. 68
9 Lumineszenz ................................................................................................................... 69
9.1 Messmethoden ............................................................................................................................... 69
9.2 Lumineszenz Spezifikationen .......................................................................................................... 70
9.2.1 Allgemeine Spezifikationen ................................................................................................ 70
9.2.2 Leistungsspezifikationen .................................................................................................... 71
9.3 Qualitätskontrolle des Lumineszenz Moduls ................................................................................... 71
9.3.1 Regelmäßige Qualitätskontrolltests ................................................................................... 71
9.3.2 ATP Detektionslimit in 384-Well Platten ............................................................................. 72
9.3.3 ATP Detektionslimit in 1536-Well Platten ........................................................................... 73
10 Alpha Technologie ........................................................................................................... 75
10.1 Grundlagen ..................................................................................................................................... 75
10.2 Alpha Modul .................................................................................................................................... 75
10.2.1 Filter ................................................................................................................................... 75
10.2.2 Optik .................................................................................................................................. 75
10.2.3 Laser .................................................................................................................................. 76
10.2.4 Detektion ............................................................................................................................ 76
10.2.5 Temperatur Korrektur ......................................................................................................... 77
10.3 Definition von Alpha Messungen ..................................................................................................... 77
10.4 Optimierung von Alpha Technologie-basierten Messungen ............................................................ 77
10.4.1 Integrationszeit .................................................................................................................. 77
10.4.2 Anregungszeit .................................................................................................................... 78
10.4.3 Dunkle Abdeckungen als Lichtschutz ................................................................................ 78
10.5 Alpha Spezifikationen ...................................................................................................................... 78
10.5.1 Allgemeine Spezifikationen, Leistungsspezifikationen ....................................................... 78
10.6 Qualitätskontrolle des Alpha Moduls ............................................................................................... 79
10.6.1 Regelmäßige Qualitätskontrolltests ................................................................................... 79
10.6.2 Detektionslimit der AlphaScreen Omnibeads 384-Well Platte ........................................... 79
10.6.3 Uniformität der AlphaScreen Omnibeads 384-Well Platte ................................................. 81
11 Absorption ...................................................................................................................... 83
11.1 Messtechniken der Absorption ........................................................................................................ 83
11.1.1 Absorption .......................................................................................................................... 83
11.1.2 Absorptionsscan ................................................................................................................ 83
11.2 Küvetten Modul ............................................................................................................................... 83
11.2.1 Küvetten Optik ................................................................................................................... 83
11.3 Messmittel ....................................................................................................................................... 84
11.3.1 Mikroplatten ....................................................................................................................... 84
11.3.2 Küvetten Adapter ............................................................................................................... 84
11.3.3 Küvetten Öffnung ............................................................................................................... 85
2021-06 Gebrauchsanweisung - Grundlagen - SPARK - Artikel Nr. 30124652 - Version 2.0 7