Filtration - Sartorius Sartocon Slice Self Contained Gebrauchsanleitung

6

Filtration

A critical moment for tangential flow filtration is at start up. The medium to
be filtered reaches a clean membrane surface. It has not yet formed a gel
layer (secondary layer), and the speed at which the retentate flow passes
the membrane is still not constant or sufficient for TFF conditions.
CAUTION
Risk of injury and property damage caused by overpressure!
The pressure of the inlet P
Contained cassettes up to 1.4m² are qualified to withstand a pressure of 4 bar.
— The cassettes may be damaged if the pressure is higher.
IMPORTANT
The backpressure on the permeate side must not exceed 0 .5 bar for
Hydrosart Membrane!
®
To start the filtration process, always proceed as follows:
Make sure that the permeate area is filled with one of the liquids listed
t
below:
— the final rinse water
— isotonic saline solution.
To prime the system using one of the liquids listed above, follow the
directions given in (Chapter "5.3.1 Rinsing Cassettes", page 21).
At the beginning of the filtration process, partly close the permeate
t
outlets.
Introduce the process fluid into the system. Generate the desired
t
TFF rate.
Open the permeate outlet valve until the desired TMP is reached.
t
Make sure that the desired TFF rate for the process is maintained.
t
— Ensure that the TFF rate is sufficient.
— If the transmembrane pressure (TMP) is low, ensure that a high
TFF rate is set.
For information about the reference values for your solution, ask our
t
application specialists.
During filtration, the crossflow rate should be held constant. If viscosity
increases during filtration, ensure to keep the crossflow rate constant to
avoid gel layer formation.
Instructions for Use Sartocon
must not exceed 3 bar. All SFM-OP-144... Self
feed
Slice Self Contained | Sartocube
®
Filtration
Self Contained 23
®