Performance Parameters - Leica DMLS Bedienungsanleitung

Performance parameters

Due to basic physical principles and the
physiology of the human eye, all imaging
techniques, not only the microscope, are
subject to limitations in performance. For proper
use of the DM LS microscope you should
therefore know and observe the following
information.
Tube length
The DM LS microscope series is based on tube
length ∞ (infinity) and a focal length of the tube
lens of f = 200 mm. Therefore, only objectives
with the engraving ∞ (Fig. 21) and M25 thread
may be used.
Objective lettering
Examples (see also Fig. 21) and meaning of the
symbols:
∞/–
C PLAN 10 x/0.22 C PLAN 40 x/0.65 N PLAN 50 x/0.75
Fig. 21 Examples of objectives
1 Brightfield objective, 2, 3 POL objectives, 4 Phase contrast immersion objective, 5 Immersion objective with iris diaphragm,
6 CORR objective for inverted microscopes
Some immersion objectives with a knurled ring have front part which can be pushed up and "locked" with a small rotational
movement. This device must be unlocked for observation! The sleeve of PL FLUOTAR and PL APO objectives can be rotated so
that the engraving can be read more easily.
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∞/0.17 ∞/0/D
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∞
Objective for infinite tube length (∞).
–
The objective can be used with and without
coverglass.
0.17
The objective may only be used with a cover-
glass of the standard 0.17 mm thickness. Use
without coverglass or with a coverglass of a
very different thickness will result in a distinct
drop in performance, especially for objectives
with high apertures.
0
Use without a coverglass, e. g. for cell smear
specimens.
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