4. Fill two empty 100 mm-style Petri dishes with 20–25 mL of sterile purified water.
5. Place the dispenser over the dish containing Lysol solution.
6. Push the dispensing knob down until the tampers are released. Hold the knob in the depressed condition for 30 sec.
7. Repeat steps 5 and 6 with the isopropyl alcohol solution.
8. Repeat steps 5 and 6 with each of the dishes of purified water. This will help ensure that the cleaning agents have been
completely removed.
The tampers have now been cleaned and decontaminated. Any remaining alcohol and water left on the tampers will evaporate. The unit may
be stored, or if testing has not been completed, dry the tampers by releasing them several times (depressing the knob fully) while the unit
is placed over a sterile towel, gauze pad, swab, or other sterile absorbent material. The tampers can also be allowed to air dry, but this will
require additional time, depending on the temperature and relative humidity in the area in which the dispenser is being stored.
TROUBLESHOOTING GUIDE
6-, 8-, and 12-Place
Read the insert before operating any BD BBL Sensi-Disc Dispenser.
If one or more discs fail to dispense:
1. Ensure the operating knob is UP.
2. Ensure the lock knob is on "Unlock."
3. Examine for empty or damaged cartridges.
4. Examine for loose discs.
To clear loose or jammed discs:
1. Remove cartridges.
2. Set lock knob on "Lock."
3. Push dispensing knob several times.
4. Examine bottom for trapped discs; if present, place inverted cartridges in appropriate positions and operate dispenser over a
sterile surface.
SERVICE AND MAINTENANCE INFORMATION
6-, 8-, and 12-Place
Should any malfunction occur that cannot be corrected by the "CLEARING PROCEDURE," "CLEANING AND DECONTAMINATION
PROCEDURE," or "TROUBLESHOOTING GUIDE," in the United States, telephone Technical Services for instructions at 1.800.638.8663.
For International locations, please contact your local BD Representative.
REFERENCES
1. Bauer, A.W., W.M.M. Kirby, J.C. Sherris, and M. Turck. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J.
Clin. Pathol. 45: 493–496.
2. Ryan, K.J., F.D. Schoenknecht, and W.M.M. Kirby. 1970. Disc sensitivity testing. Hospital Practice. 5: 91–100.
3. Food and Drug Administration. Standardized disc susceptibility test. Section 460.1, Title 21 of the Code of Federal Regulations.
4. National Committee for Clinical Laboratory Standards. 1997. Approved Standard M2-A6, Performance standards for antimicrobial disk
susceptibility tests, 6th ed. National Committee for Clinical Laboratory Standards, Wayne. Pa.
5. National Committee for Clinical Laboratory Standards. 2003. Approved Standard: M2-A8. Performance standards for antimicrobial disk
susceptibility test, 8th ed. NCCLS, Wayne, Pa.
6. Jousimies-Somer, H.R., P.H. Summanen, and S.M. Finegold. 1999. Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and other
anaerobic gram-nagative rods and cocci, p. 690–711. In P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover, and R.H. Yolken (ed.), Manual
of clinical microbiology, 7th ed. American Society for Microbiology, Washington, D.C.
7. Summanen, P., E.J. Baron, D.M. Citron, C.A. Strong, H.M. Wexler, and S.M. Finegold. 1993. Wadsworth anaerobic bacteriology manual,
5th ed. Star Publishing Company, Belmont, Calif.
Technical Information: In the United States contact BD Technical Service and Support at 1.800.638.8663 or www.bd.com.
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