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7.2 Calibration counter
1. Select Menu > Options > Calibrate > Counter to show on the display the number of pipettings
since the last calibration. The counter is reset to zero when the calibration is performed.
7.3 Checking the calibration
The pipette calibration is checked with the maximum volume (nominal volume) and with the minimum
volume. A new tip is first pre-wetted 3 to 5 times and a series of ten pipettings are done with both
volumes. A pipette is always adjusted for delivery (Ex) of the selected volume. Measuring volumes
taken from balance is not allowed.
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the accuracy (A) and precision (cv) of both series.
4. Compare the results to the limits in
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
Table 2 Maximum permissible errors according ISO 8655
Range Volume (μL)
0.2–5 mL
7.4 Adjustment
7.4.1 Two point calibration
The two-point calibration is the standard calibration.
1. Do the pipetting series with both maximum and minimum volumes.
2. Calculate the actual results.
3. Select Menu and select Options with the scroll key and accept with OK.
4. Select Calibrate and accept with OK.
5. Select Two points and confirm with OK. The target maximum and target minimum volumes are
shown on the display.
6. Select Edit and change the actual maximum volume with the scroll key and accept with OK.
7. Change the actual minimum volume with the scroll key and accept with OK.
8. Save? is displayed. Accept with Yes.
The adjustment has been changed.
7.4.2 One-point calibration
The one-point calibration can be used if a single specific volume must be calibrated. The calibration
volume can be selected from the entire volume range. Note that the inaccuracy of other volumes
changes also and the performance for other volumes cannot be assured.
1. Do the pipetting series with the calibration volume.
2. Calculate the results.
3. Select Menu and select Options with the scroll key and accept with OK.
4. Select Calibrate and accept with OK.
5. Select One point and confirm with OK. The calibration volume is shown on the display.
6. Select Edit and change the calibration volume with the scroll key and accept with OK.
14 English
Table 2.
5000 ±40.0 µL
200 ±40.0 µL
Accuracy
±0.8 %
±20.0 %
Precision
15.0 s.d. µL 0.3 cv%
15.0 s.d. µL 7.5 cv%