Operating Information
IMPORTANT
28
Operating Instructions BioPAT
5.2.2
In-process Measurement
Temperatures in the exhaust air >40°C or
oxygen-free exhaust air can damage the sensors
in the BioPAT
Xgas!
®
The pure nitrogen used during zero-point cali-
bration of the DO sensor in the culture vessel
must not escape into the BioPAT
and condensation in the exhaust air can damage
the sensors.
Ensure that exhaust cooling is always active
t
during measurement. Monitor whether
condensation forms in the exhaust line. Abort
the measurement if condensation enters
the BioPAT
Xgas. Dry the BioPAT
®
described below [→ Troubleshooting].
Bioreactors Biostat
After autoclaving, set up the culture vessels
t
at the work place. If required, calibrate the
zero point of the built-in DO sensor with pure
nitrogen.
Ensure that the process temperature is
t
< 40°C.
Connect the exhaust line to the BioPAT
t
Xgas.
Xgas
®
Xgas. Humidity
®
®
B or B-DCU II
®
Xgas as
®