Sartorius BioPAT Xgas Betriebsanleitung Seite 27

Fig. 6: 1-point calibration
Perform 1-point calibration during initial start-
t
up and always when the analyzer indicates
you do so. Disconnect the connection or lines
from the bioreactor (exhaust air filter) and
channel fresh air (0.04% CO
volume, residual N
200 ml/min.Observe the specifications in the
"Data Sheet". Flushing air with a different
composition will falsify later measurements.
Press the two buttons on the BioPAT
t
5 seconds.
As soon as the left LED is lit up continuously
t
in green, the analyzer is ready for operation.
For autoclavable bioreactors:
When disconnecting from the exhaust line
during preparation of the bioreactor for the pro-
cess, e.g. for autoclaving, you must not switch off
the BioPAT Xgas. The displayed measurement
®
signal then indicates the measurement of the
ambient air.
On bioreactors with autoclavable culture
t
vessels, remove any exhaust line already
connected to the BioPAT
Prepare the bioreactor for the process
t
[→ Operating instructions for the bioreactor],
e.g. sterilization, calibration of the DO sensor
and inoculation.
Operating Instructions BioPAT
Operating Information
, 20.97% O
2
) at a flow rate of min.
2
Xgas.
®
Xgas
®
by
2
Xgas for
®
27