Cleaning The Flow Cell - Buchi UV Monitor C-630 Handbuch

Büchi UV Monitor C-630
SOP 2
SOP 3
6. Put the lid back on from the top of the instrument and fasten the
screws of the cover.
7. Check the intensity values in the INT-menu.
Hint: If a new lamp is installed, it takes about 24 hours to reach
the optimum working conditions.
Changing the halogen tungsten lamp.
This instruction applies to the UV Monitor C-630.
1. Unscrew the housing and remove it by lifting.
2. Pull the 2-pole plug of the old lamp (item 1T). Unscrew the two
screws in the lamp socket (item 2) with a screw driver and
remove the whole lamp from the instrument including its socket
(item 4) and cables.
3. Loosen the pin screw (item 5) and pull the lamp out of its socket.
4. Insert the new lamp assuring that it is correctly seated in the
guiding slot (item 5, 5a) and fasten the pin screw.
5. Insert the socket with lamp into the instrument, screw it in
securely (item 2), and connect the 2-pole plug into the 2-pole
socket (item 1T).
6. Increase the lamp counter in the GLP-menu. The operating time
counter will be set to zero by this implementation.
7. Put the lid back on from the top of the instrument and fasten the
screws of the cover.
8. Check the intensity values in the INT-menu.

Cleaning the flow cell

Noisy baselines and low sensitivities may be due to a dirty flow cell.
This may also be indicated by a low value for sig in the signal menu
when flushing the cell with pure solvent. In most cases it is sufficient to
purge the flow cell according to the following SOP.
Purging the Flow Cell
1. Purge the flow cell using one of the following solvents: sodium
dodecyle sulfate (SDS), 1m HCl, 1m NaOH, ethanol, or acetone.
2. Run the solvent through the flow cell using a syringe and leave for
approximately 5 minutes..
3. Rinse extensively with water and then blow dry using a gentle
stream of pure nitrogen.
Never dry with compressed air from a „house" line as this will
contain microdroplets of oil that will coat the cell.
When the optics module is not in use, disconnect the flow cell and clean
out traces of salt and protein with a syringe filled with distilled water.
Before storing the flow cell inject a dilute solution (10-25%) of ethanol or
i-propanol to prevent microbial growth.
In case the flow cell purging do not provide sufficient success, all flow
cells can easily be disassembled for cleaning the lenses.
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