Installation Of The Flow Cell; Simple Maintenance; Control Of The Lamp's Functionality - Buchi UV Monitor C-630 Handbuch

Büchi UV Monitor C-630 11

Installation of the Flow Cell

The UV Monitor C-630 comes factory configured without a flow cell. The
device is equipped with a "dummy" cell which does not have any optical
parts.
The instrument or the lamp are not needed to be switched off for
changing the flow cell. When the flow cell is removed a error message
intens.overflow will appear in the upper display line because the
analog to digital converter gets an overflow due to the incoming light.
This is not an instrumentation fault! After having installed the new
flow cell properly, press any button to delete the error message.
Column Pump Head
Sample
Waste
Flow Cell
Eluent
Waste
Fig. 6 Isocratic MPLC System

Simple Maintenance

Control of the lamp's functionality

The deuterium lamp used with the UV Monitor C-630 has an extended
life time to ensure long-time functionality and reliable measurements
with low noise and baseline drift as well as high sensitivity. The actual
using time of the lamp depends on different factors, like the number of
lamp starts, the average burning time and your requirements
concerning noise and sensitivity.
To check the functionality of the lamp, the two intensity values sig and
ref to be found in the SIGNAL menu on page 8 provide helpful
information. The ref value refers to the intensity of the light measured in
the reference channel and can be used for checking the quality of the
lamp.
On delivery of the photometer, the sig.-value is set to the range of 0.39
to 0.78 and the ref.-value to 0.2 to 0.4, measured with the dummy cell at
a wavelength of 254 nm.
We recommend to check the ref value at regular intervals under the
conditions mentioned above (dummy cell, λ = 254 nm). This applies
especially in case higher noise levels or decreased sensitivity are
observed on working with the UV Monitor C-630. In these observations