Other Operating Tips
• Columns should not be maintained at elevated pH or elevated
temperature when not in use (see Table 2).
• New columns may contain a mixture of organic solvents and water,
which may contain buffer salts. See Table 3 for details of the shipping
solvents. Initially, care should be taken not to pass any mobile phase
through the column that may cause a precipitate to form or may not
be fully miscible.
• Agilent BioHPLC and AdvanceBio columns are compatible with all
common eluents used for analysis of biomolecules.
Table 1. Maximum operating parameters – columns
up to 4.6 mm
Column
AdvanceBio Peptide Mapping
AdvanceBio Peptide Plus
AdvanceBio Oligonucleotide
AdvanceBio Amino Acid Analysis
AdvanceBio RP-mAb
ZORBAX 300SB
Poroshell 300
ZORBAX RRHD
PLRP-S
(continued)
Particle size
2.7 µm
3.5 µm
3.5 µm, 5 µm, 7 µm
5 µm
1.8 µm
3 µm
5 µm, 8 µm, 10 µm
10-15 µm, 15-20 µm, 30 µm
ENGLISH
Pressure limit
600 bar
400 bar
1200 bar
275 bar
207 bar
103 bar