Agilent BioHPLC Benutzerinformation Seite 6

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Table 2. Column operating parameters – pH and temperature
Column
AdvanceBio Peptide Mapping
AdvanceBio Peptide Plus
ZORBAX RRHD
ZORBAX 300SB-C8, C3, CN
ZORBAX 300SB-C18
AdvanceBio RP-mAb
Poroshell 300
Poroshell 300 Extend-C18
AdvanceBio Oligonucleotide
AdvanceBio Amino Acid Analysis
PLRP-S
Note: All silica-based packings have some solubility in pH >6 aqueous mobile phases. When using silica-
based columns at pH >6, best column lifetime is obtained at lower temperatures (40 °C max) using low buffer
concentrations in the range of 0.01 to 0.02 M.
Table 3. Shipping solvents
Column
AdvanceBio Peptide Mapping
AdvanceBio Peptide Plus
ZORBAX RRHD
ZORBAX 300SB
AdvanceBio RP-mAb
Poroshell 300 and 300 Extend
AdvanceBio Oligonucleotide
AdvanceBio Amino Acid Analysis
PLRP-S
Recommended
pH range
2.0-8.0
1.0-8.0
1.0-8.0
2.0-11.0
3.0-11.0
1.0-14.0
Shipping solvent
Mixture of acetonitrile
and water
Mixture of acetonitrile
and water
Mixture of methanol
and water
Mixture of acetonitrile
and water
7:1 acetonitrile/water
Maximum operating
temperature
60 ºC
80 ºC
90 ºC
60 °C below pH 8,
40 °C above pH 8
65 ºC
200 ºC
Compatibility
Water and all common organic
solvents.
Water and all organic solvents,
including N,N-dimethylformamide
and dimethylsulfoxide.
Water and all common organic solvents.
Modifiers including HFIP and TEAA.
Aqueous organic solvents
including N,N-dimethylformide and
dimethylsulfoxide. 100% aqueous is
not recommended as it will reduce
column performance and lifetime.