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other operating tips
• While generally not harmful to the column, reverse flow should be
avoided except to attempt removal of clogged frit (see "column care").
• Always use high purity reagents and chromatography grade solvent
to prepare your mobile phase. Degas and filter all mobile phase prior
to use.
• Disassembling a column will degrade column performance.
• New columns contain a mixture of organic solvents and water. See
your QC Performance Report for the solvent composition in your
column. Initially, care should be taken not to pass any mobile phase
through the column that may cause a precipitate to form.
• Agilent reversed-phase columns are compatible with water and all
common organic solvents.
• The use of a guard column is recommended to protect your column
and increase its lifetime.
• Columns should not be maintained at elevated pH or elevated
temperature when not in use.
• Avoid use of this column outside of recommended pH ranges for
column phase (see next page). Expect reduced lifetime when operating
outside the recommended pH and temperature ranges.
If you are using Poroshell 120 or ZORBAX RRHT or RRHD
columns, 1.8 µm, consider using a Fast Guard for UHPLC to
protect your analytical column.
See agilent.com/chem/fastguards for more information.
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