Filtration - Sartorius Stedim Biotech Sartocon Slice Installationsanleitung

5. Filtration

The start of crossflow filtration is a critical time. The medium to be filtered reaches
a clean membrane surface. It has not yet formed a covering layer (secondary layer),
and the speed at which the retentate flows past the membrane is still not constant or
sufficient for crossflow conditions.
Risk of injury and property damage caused by overpressure!
For standalone units, the pressure at the retentate inlet may not exceed 4 bar (P
and 3 bar at bag loop assemblies!
− The cassettes may be damaged if the pressure is higher.
The pressure on the permeate side (P
membranes!
To filter, complete the following steps:
Make sure that the permeate area is filled with one of the liquids listed below:
t t
− the final rinse water
− isotonic saline solution.
To prime the system using one of the liquids listed above, follow the directions given
in the section entitled "Rinsing Cassettes".
At the beginning of the filtration process, close the permeate outlets.
t t
Introduce the process fluid into the system. Generate the desired crossflow rate.
t t
Open the permeate outlet valve until the desired transmembrane pressure (TMP) is
t t
reached [Æ Chapter "4.2 Notes on System Setup", page 9].
Make sure that the desired crossflow rate for the process is maintained.
t t
− Ensure that the crossflow rate is sufficient.
− If the transmembrane pressure (TMP) is low, ensure that a high crossflow rate is
set.
For information about the reference values for your solution, ask our applications
t t
specialists.
The crossflow rate should be kept constant during filtration. If, during the course of
filtration, the viscosity of the fluid increases, P
consistent cleaning effect for the membrane surface.
) may not exceed 0.5 bar for Hydrosart®
perm
must be increased. This ensures a
feed
)
feed
Filtration 13