Operation; Setting The Volume; Preparation For Operation; Pipetting Recommendations - VWR Ultra High-Performance VE2 Handbuch

2. OPERATION

2.1. SETTING THE VOLUME

Setting the volume is performed by the volume adjust-
ment knob (fig. 1B).
A B
Fig. 2
The range of aspirated volume is displayed on the
counter cap (fig.1G). The volume is displayed by the
counter and consists of four digits, which should be read
from top to bottom. The digits on the lower drum consti-
tute minimum graduation for the particular model. Value
of the minimum graduation (increment) is presented in
Table 1.
Volume may be changed after setting the knob in its top
position (fig.2B).
After setting the desired volume, the knob (fig.1B) should
be locked by pushing into the lower position (fig. 2C).
Examples of counter indications:
0.1-2 μl 2-20 μl
0.1 μl 2 μl 2 μl
Counter indications above the horizontal indicators
shown volume in [μl].
To achieve maximum accuracy, the required volume
should be set from higher volume by reducing indica-
tions of the counter.
5
C
100-1000 μl
20 μl 100 μl 1000 μl
Example of correct volume setting:
• If the required volume is lower than
the volume set on the counter it is
necessary to reduce indications of
the counter to the required value.
Before achieving the required value it
is necessary to reduce the speed
with which you rotate the adjustment
knob (fig.1B), so as not to exceed the
value (fig.3).
• If the required volume is higher than the volume set on
the counter it is necessary to increase indications of the
counter to the value which exceeds the required vol-
ume by rotating the bottom drum by about 1/3 of its cir-
cumference. Then, by slowly rotating the adjustment
knob (fig. 1B), reduce the setting to the required value
(fig. 3).
After setting the required volume, the knob (fig. 1B) should
be moved downward to lock the set volume (fig. 2C).

2.2. PREPARATION FOR OPERATION

Place a tip on the shaft of the pipettor. See Table 1 for
the appropriate tip designed for the given pipettor
model. Firmly press the tip on the shaft using twisting
motion to ensure airtight seal.
CAUTION:
• It is recommended to use the tips recommended by
the manufacturer, as only those tips will ensure accu-
rate and repeatable liquid measurements.
• Do not place tips by applying swinging motion, as this
can damage the shaft and the plunger. It is particularly
applied to small-volume pipettors.
• Never aspirate liquid into a pipettor without a tip
attached.

3. PIPETTING RECOMMENDATIONS

Observing the following recommendations will ensure
accuracy and repeatability of liquid sampling.
• While operating VWR UHP pipettor the volume setting
should be locked - the adjustment knob in its lower
position (fig. 2C).
ENGLISH
Fig. 3
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