Calibration - Thermo Scientific Finnpipette Handbuch

3. Deliver the preset volume by gently depressing the push button to the first stop.
Hold the push button at the first stop. Some liquid will remain in the tip and this
should not be included in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into the
container.
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REPETITIVE TECHNIQUE
The repetitive technique offers a rapid and simple procedure for repeated delivery of
the same volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm,
and slowly release the push button. This action will fill the tip. Withdraw the tip
from the liquid touching against the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing the push button to the first stop.
Hold the push button at the first stop. Some liquid will remain in the tip and this
should not be included in the delivery.
4. Continue pipetting by repeating steps 2 and 3.
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PIPETTING WHOLE BLOOD
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with blood.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress the push button to the first stop,
making sure the tip is well below the surface.
2. Release the push button slowly to the ready position. This will fill the tip. Keep the
tip in the solution.
3. Depress the push button to the first stop and release slowly. Keep repeating this
procedure until the interior wall of the tip is clear.
4. Finally, depress the push button all the way to the second stop to completely empty the tip.
All Finnpipettes are factory calibrated and adjusted to give the volumes as specified
with distilled or deionized water. The pipettes are constructed to permit re-adjustment
for liquids of different temperature and viscosity.
DEVICE REQUIREMENTS AND TEST CONDITIONS
An analytical balance must be used. The scale graduation value of the balance should
be chosen according to the selected test volume of the pipette:
Volume range
under 10 µl
10-100 µl
above 100 µl
Test liquid: Water, distilled or deionized, "grade 3" water conforming ISO 3696. Tests
are done in a draft-free room at a constant (±0.5°C) temperature of water, pipette and
air between 15°C to 30°C. The relative humidity must be above 50%. Especially with
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CALIBRATION

graduation
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