Pipetting Techniques - Thermo Scientific Finnpipette Handbuch

SAFETY LABEL
You can mark the pipette application your initials the calibration date, etc. on the safety label.
Remove the clear plastic window on the edge closest to the push button (use the
service tool that comes with the pipette, or a screwdriver). Mark the adhesive label
with a felt-tipped or other pen and snap the window back in place.
SHELF HANGER
You can attach the pipette shelf hanger on a counter, pipette stand or anywhere where
you want to hang your pipette.
Clean the area where you plan to attach the shelf hanger. Apply two stickers to the
underside of the shelf hanger. Press the shelf hanger firmly into place -- on a shelf,
countertop or pipette stand. To use, hang the grippy finger rest on the shelf hanger.
Push and release the push button slowly at all times,
particularly when working with high viscosity liquids.
Never allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone.
Check for foreign particles in the tip.
Before you begin your actual pipetting work, fill and empty the tip 2-3 times with the
solution that you will be pipetting. Hold the pipette in an upright position while aspirating
liquid. The grippy should rest on your index finger. Make sure that the tips, pipette and
solution are at the same temperature.
FORWARD TECHNIQUE
Fill a clean reagent reservoir with the liquid to be dispensed.
1 Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm
and slowly release the push button. Withdraw the tip from the liquid touching it
against the edge of the reservoir to remove excess liquid.
3. Deliver the liquid by gently depressing the push button to the first stop. After a
delay of about one second, continue to depress the push button all the way to the
second stop. This action will empty the tip.
4. Release the push button to the ready position.
If necessary, change the tip and continue pipetting.
REVERSE TECHNIQUE
The reverse technique is suitable for dispensing liquids that have a high viscosity or a
tendency to foam easily. The technique is also recommended for dispensing very
small volumes.
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm,
and slowly release the push button. This action will fill the tip. Withdraw the tip from
the liquid touching it against the edge of the reservoir to remove excess liquid.
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PIPETTING TECHNIQUES

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Figures 3-6:
A = Ready position
B = First stop
C = Second stop
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