Easy Maintenance; Piston Backflushing; Changing And Cleaning The Pump Head - Knauer WellChrom HPLC Pump K-1001 Handbuch

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Easy Maintenance

Piston backflushing

SOP 23 Piston backflushing

Changing and Cleaning the Pump Head

SOP 24 Removing the Pump Head
You may carry out maintenance and cleaning only on the pump head.
Both pump heads (10 ml and 50 ml) differ only in some sizes. Therefore the
descriptions are valid for both of them. If you should use some older pump head
versions (stainless steel or PEEK), you should contact our service division to
get the corresponding instructions.
Backflushing the piston removes traces of salt and other decontaminates from
the backside of the pistons.
If you use saline solvents or buffer solution we highly recommend that you
continuously backflush in order to prevent crystallization which can damage
your piston seals.
1. Push a 1/16 ID tube onto both flushing opening, see Piston
backflushing capillaries, Pos. {3.1} in Fig. 3 on page 9.
2. Place the low end of the tubing in a flask.
3. Fill the syringe with rinsing liquid.
4. Connect the syringe with the tubing.
5. Press liquid through the pump head, until it flows without any air
bubbles into the container.
6. Remove tubings from flush opening.
We recommend connecting both flush openings with a tubing to prevent
vaporization of solvents and drying out of the piston chamber.
If you want operation with continuous backflushing you can attach two
containers of rinsing liquid instead of the priming syringe. The containers should
be positioned that one container is located higher than the other, thus ensuring
liquid flow through the pump head without any assistance.
This SOP refers to the HPLC Pump K–1001.
For exchange of the pump head, or for disassembling it in order to clean valves
or replace piston seals, piston rods etc., the pump head can easily be removed.
1. Purge the pump head with a suitable cleaning agent and then with
distilled water. You can use SOP 11 „Purging the HPLC Pump K–1001"
on page 19.
If organic solvents remain in the pump head, danger of skin irritation may
exist.
2. Remove the two solvent tubings from the „Eluent inlets", Pos. {3.2}see
Fig. 3 on page 9.
3. Loosen the „Eluent outlet to transducer", Pos. {3.7} and the „Inlet to
transducer", Pos. {4.1}.
Easy Maintenance